Additional endocrine disruption screening services are available. Cyprotex and its partners are also able to offer the full range of EDSP series 890 protocols.
The AR is a ligand-dependent transcription factor that controls the expression of specific genes. The binding of the AR to its native ligands 5α-dihydrotestosterone (DHT) and testosterone initiates male sexual development and differentiation.
2Tan MHE et al., (2015) Androgen receptor: structure, role in prostate cancer and drug discovery. Acta Pharmacologica Sinica 36: 3-23
|Test Method||AR Redistribution Assay (ThermoScientific)|
|Cell Type||Recombinant U2OS cells stably expressing human androgen receptor|
|Test Article Concentrations||10 µM in quadruplicate (alternative concentrations or dose curves available on request)|
|Test Article Exposure||Overnight|
|Reference Controls||Dihydrotestosterone (agonist)
|Typical Vehicle Control||0.5% DMSO|
|Analysis Method||ThermoScientific ArrayScan® VTi High Content Imaging|
|Data Delivery||% Effect compared to control (single concentration)
EC50 (dose response)
1 Hua Y, et al, (2014) High-content positional biosensor screening assay for compounds to prevent or disrupt androgen receptor and transcriptional intermediary factor 2 protein-protein interactions. ASSAY Drug Dev Technol 12(7); 395-418
2 Tan MHE et al., (2015) Androgen receptor: structure, role in prostate cancer and drug discovery. Acta Pharmacologica Sinica 36; 3-23