Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been adopted as gene-targeting tools. ZFN-induced double strand breaks are subject to cellular DNA repair processes that lead to both targeted mutagenesis and targeted gene replacement at remarkably high frequencies.
1Carroll D (2011) Genetics 188; 773-782
|Test Article Concentration||10 μM|
|Transporter Systems Available||Parental Caco-2 derived cell line (C2BBe1) and MDR1, BCRP and MRP2 single and double knockout cell lines|
|Period of Cell Culture||20-22 days|
|Number of Replicates||2|
|Incubation Time||2 hr|
|Integrity Marker||Lucifer Yellow|
1 Carroll D (2011) Genetics 188; 773-782
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