Understand if your compound is metabolized by aldehyde oxidase (AO).
Aldehyde oxidase (AO) reaction phenotyping is a non-CYP mediated metabolism assay within our portfolio of in vitro ADME screening services. Cyprotex deliver consistent, high quality data with the flexibility to adapt protocols based on specific customer requirements.
It has been increasingly recognized in this past decade that AO, through its unique structure, distribution, and substrate recognition, has an important role to play in the metabolism of drugs.
1Pryde DC et al. (2010), J. Med. Chem., 53:8441-8460
|Test System||Human liver cytosol incubated with and without specific AO inhibitor, 100 µM menadione (other species and enzyme sources available on request)|
|Test Article Concentration||1 µM (different concentrations available)|
|Positive Control Substrate||Phthalazine|
|Time Points||0, 10, 20, 40, 60, 120 min|
|Test Article Requirements||100 µL of a 10 mM DMSO solution (or equivalent amount in solid)|
|Data Delivery||% Parent compound remaining at each time point
Standard error of half life
1Pryde DC et al. (2010) Aldehyde oxidase: an enzyme of emerging importance in drug discovery. J Med Chem 53: 8441-8460
Learn more about drug metabolism in chapter 3 of our popular Everything you need to know about ADME guide.