Understand if your compound is metabolized by MAO-A or MAO-B enzyme.
Monoamine oxidase (MAO) reaction phenotyping is a non-CYP mediated metabolism assay within our portfolio of in vitro ADME screening services. Cyprotex deliver consistent, high quality data with the flexibility to adapt protocols based on specific customer requirements.
In human brain the predominant form is MAO-B, expressed at highest levels in astrocytes and serotonergic neurons, while MAO-A is expressed at highest levels in catecholaminergic neurons.
1Hotamisligil GS and Breakefield XO (1991) Am J Hum Genet 49(2); 383–392
|Test System||hMAO-A and hMAO-B expressed enzyme|
|Test Article Concentration||1 µM (different concentrations available)|
|Time Points||0, 5, 15, 30, 45 min|
|Negative Control||cDNA expressed control preparation (no MAO present)|
|Test Article Requirements||100 µL of a 10 mM DMSO solution (or equivalent amount in solid)|
|Data Delivery||% Parent compound remaining at each time point for each isoform
Standard error of half life
1 Hotamisligil GS and Breakefield XO (1991) Human monoamine oxidase A gene determines levels of enzyme activity. Am J Hum Genet 49(2); 383–392