A Comprehensive Comparison of In Vitro Assays for Detecting Mitochondrial Toxicity

Mitochondria are often described as the “powerhouses” of cells. It is not surprising, therefore, that drug-induced mitochondrial dysfunction is a key mechanism of toxicity, especially in organs which are highly dependent on energy production such as the heart, liver, brain and skeletal muscle.

There are various in vitro techniques available for assessing mitochondrial dysfunction. These range in complexity as well as predictivity. Our research has focused on a number of these techniques including:

  • Cytotoxicity assay in the presence of glucose and galactose media
  • High content imaging (mitochondrial membrane potential and cytotoxicity)
  • Mitochondrial respiration (cellular oxygen consumption rate, extracellular acidification rate and reserve capacity using the Seahorse platform)

A comprehensive comparison of these methods was performed to assess their ability to predict drug-induced mitochondrial toxicity for a set of reference drugs.

You can find out more about our research in drug-induced mitochondrial toxicity by downloading our recent poster, which was presented at the 54th Congress of the European Societies of Toxicology (EUROTOX) from the 2nd-5th September 2018 in Brussels.

Download the poster

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