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In vitro toxicology

High Content Imaging of 3D Microtissues

Background information

  • The environment created by a 3D culture model allows reconstitution of the natural cellular physiology by promoting the complex cell-cell and cell-matrix network communications found in vivo.
  • For imaging purposes, microtissues are formed using round bottom ultra-low adhesion cell culture plates which promote aggregation of cells into functional 3D microtissues.
  • Microtissues can be formed from single or co-cultured cell populations and their longevity permits use in long-term repeat dose toxicity studies to investigate any cumulative effect.
  • The combination of biologically relevant in vitro 3D cell-based models with multiparametric HCS assays presents a viable screening strategy for the detection of novel therapeutics that cause toxicity early in drug development.

Protocol

High content imaging of 3D microtissues

Microtissues* HepaRG, liver, spontaneously beating cardiac (mono-and co-cultures available)
Endpoints* GSH content, ROS formation, phospholipidosis, steatosis, mitochondrial membrane potential, ATP content, LDH release
Time Points* 4 hr, 16 hr, 72 hr and 14 day repeat dose

*others available on request

Data

Data from Cyprotex's 3D microtissue high content imaging assays

 


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