ATP Content in 3D Microtissues

Background information

• The environment created by a 3D culture model allows reconstitution of the natural cellular physiology by promoting the complex cell-cell and cell-matrix network communications found in vivo.
• Different scaffold-free 3D microtissue models are available at Cyprotex including spheroids produced using Ultra-low adhesion plates or using hanging drop technology.
• Microtissues can be formed from single or co-cultured cell populations and their longevity permits use in long-term repeat dose toxicity studies to investigate any cumulative effect.
• ATP content is a biochemical endpoint used to measure general cell viability. This can be incorporated with LDH release to monitor cell death over time.