Understand the potential for phototoxicity following topical exposure of your individual ingredients, chemicals or pharmaceutical products.
Phototoxicity assessment is in our portfolio of in vitro toxicology services for the cosmetics/personal care, chemical or pharmaceutical industry. Cyprotex delivers consistent, high quality screening data under non-GLP conditions.
The in vitro 3T3 NRU phototoxicity test is based on a comparison of the cytotoxicity of a chemical when tested in the presence and in the absence of exposure to a non-cytotoxic dose of simulated solar light.
1 OECD Guideline for the Testing of Chemicals 432: In Vitro 3T3 NRU Phototoxicity Test; April 2004
Models Available | Balb/c 3T3 mouse fibroblast cell line |
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Number of Replicates/Concentrations | 6 replicates at 8 concentrations of test article (3T3 cells) |
Exposure Conditions | Condition 1: 3T3 cells kept in dark chamber. Condition 2: 3T3 cells exposed to calibrated non-toxic dose of UVA. |
Assay Controls | Chlorpromazine (Positive control) Vehicle Background |
Endpoints | Neutral Red uptake (3T3 cells) |
Data Delivery | Photo irritation factor (PIF) Mean photo effect (MPE) Phototoxic category |
Phototoxic Potential Categorization | Data from in vitro 3T3 Neutral Red Uptake test |
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No phototoxicity | PIF < 2 or MPE < 0.1 |
Probable phototoxicity | PIF between 2 and 5 or MPE between 0.1 and 0.15 |
Phototoxicity | PIF > 5 or MPE > 0.15 |
1OECD Guideline for the Testing of Chemicals 432: In Vitro 3T3 NRU Phototoxicity Test; April 2004
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