Understand the potential for phototoxicity following topical exposure of your individual ingredients, chemicals or pharmaceutical products.
Phototoxicity assessment is in our portfolio of in vitro toxicology services for the cosmetics/personal care, chemical or pharmaceutical industry. Cyprotex delivers consistent, high quality screening data under non-GLP conditions.
The in vitro 3T3 NRU phototoxicity test is based on a comparison of the cytotoxicity of a chemical when tested in the presence and in the absence of exposure to a non-cytotoxic dose of simulated solar light.
1 OECD Guideline for the Testing of Chemicals 432: In Vitro 3T3 NRU Phototoxicity Test; April 2004
|Models Available||Balb/c 3T3 mouse fibroblast cell line|
|Number of Replicates/Concentrations||6 replicates at 8 concentrations of test article (3T3 cells)|
|Exposure Conditions||Condition 1: 3T3 cells kept in dark chamber.
Condition 2: 3T3 cells exposed to calibrated non-toxic dose of UVA.
|Assay Controls||Chlorpromazine (Positive control)
|Endpoints||Neutral Red uptake (3T3 cells)|
|Data Delivery||Photo irritation factor (PIF)
Mean photo effect (MPE)
|Phototoxic Potential Categorization||Data from in vitro 3T3 Neutral Red Uptake test|
|No phototoxicity||PIF < 2 or MPE < 0.1|
|Probable phototoxicity||PIF between 2 and 5 or MPE between 0.1 and 0.15|
|Phototoxicity||PIF > 5 or MPE > 0.15|
1OECD Guideline for the Testing of Chemicals 432: In Vitro 3T3 NRU Phototoxicity Test; April 2004