Detect mitochondrial toxicity of novel therapeutics using Cyprotex’s mitochondrial biogenesis assay.
Cyprotex deliver consistent, high quality data with the flexibility to adapt protocols based on specific customer requirements.
Mitochondria are the only organelles outside of the nucleus that contain their own genome and replicate itself in an independent manner from the nuclear genome
3Moreira AC et al, (2011), In: Biosensors for Health, Environment and Biosecurity, Serra PD (Ed.), 411-444
|Method||High content screening using fluorescently labelled antibodies for COX-1 and SDH-A|
|Cell Types||HepG2 or HepaRG (others available on request)|
|Exposure Time||5-8 days depending on cell line and customer needs|
|Number of Concentrations||10 concentrations (n=3 wells per concentration)|
|Data Delivery||COX-1 protein expression
SDH-A protein expression
COX-1/SDH-A protein expression ratio
Cyprotex’s mitochondrial biogenesis assay uses high content imaging to detect COX-1 (mtDNA-encoded) and SDH-A (nDNA-encoded) protein expression. Using the ratio of COX-1/SDH-A protein expression, specific effects on mitochondrial biogenesis can be identified. The positive control for the assay, chloramphenicol (antibiotic), inhibits mitochondrial biogenesis in a dose-dependent manner (figure 1).
1 Jornayvaz FR and Shulman GI (2010) Regulation of mitochondrial biogenesis. Essays Biochem 47; 69-84
2 Food and Drug Administration. Guidance for Industry: Antiviral product development - Conducting and submitting virology studies to the agency (https://www.fda.gov/downloads/Drugs/GuidanceComplianceRegulatoryInformation/Guidances/
3 Moreira AC et al, (2011) Mitochondria as a biosensor for drug-induced toxicity - Is it really relevant? Biosensors for Health, Environment and Biosecurity, Serra PA (Ed.); 411-444