Neurite outgrowth is a requisite for an accurate functional network of neurons during development. It is also crucial for neuronal plasticity, as well as neuronal regeneration.
1Salto R et al., (2015) PLoS One 10(8); e0135614
|Cell Type||Human iPSC-derived neurons (other cell types available upon request)|
|Analysis Platform||ArrayScan VTi or CellInsight CX7 (Thermo Scientific)|
|Test Article Concentrations||10 point dose-response curves in triplicate (dependent on customer requirements)|
|Quality Controls||Negative controls: 0.2% DMSO (vehicle) and chlorpromazine
Positive control: nocodazole
|Data Delivery||This assay has been optimized to assess cell health and neurite outgrowth utilizing a neuronal profiling bioapplication. Valid cell count, mean neurite average length and neurite total length per neuron are reported.|
|Valid neuron count||> 1|
|Mean neurite average length||0.04933|
|Neurite total length per neuron||0.03191|
Positive control nocodazole causes a significant decrease in mean neurite average length and neurite total length per neuron over the range of concentrations tested. Nocodazole did not have significant effects on cell viability at these concentrations.
Images show a decrease in neurite outgrowth in a dose response manner while valid cell count is not significantly affected.
|Valid neuron count||8.645|
|Mean neurite average length||8.950|
|Neurite total length per neuron||8.383|
Negative control chlorpromazine causes a significant decrease in mean neurite average length, neurite total length per neuron and valid neuron count over the range of concentrations tested. There is a direct correlation between cell loss and neurite length.
1 Salto R et al., (2015). ß-Hydroxy-ß-methylbutyrate (HMB) promotes neurite outgrowth neuro2a cells. PLoS One. 10(8); e0135614
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Min H et al, (2007). High content screen microscopy analysis of A beta 1-42-induced neurite outgrowth reduction in rat primary cortical neurons: neuroprotective effects of alpha 7 neuronal nicotinic acetylcholine receptor ligands. Brain Research. 1151; 227-235
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